GIT1 Antikörper (AA 415-736)

Produktnummer ABIN7601683

Der Kaninchen Polyklonal Anti-GIT1-Antikörper wurde für WB, ELISA, IHC, IF, ICC und FACS validiert. Er ist geeignet, GIT1 in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für GIT1 Antikörper (AA 415-736) (ABIN7601683)

Target: GIT1

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GIT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-GIT1 Antikörper

Bindungsspezifität: AA 415-736

Verwendungszweck: Anti-GIT1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-GIT1 Antibody Picoband® (ABIN7601683). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human GIT1 recombinant protein (Position: D415-Q736).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Goehler, H., Lalowski, M., Stelzl, U., Waelter, S., Stroedicke, M., Worm, U., Droege, A., Lindenberg, K. S., Knoblich, M., Haenig, C., Herbst, M., Suopanki, J., and 12 others. A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease. Molec. Cell 15: 853-865, 2004. Note: Erratum: Molec. Cell 19: 287 only, 2005. 2. Gross, M. B. Personal Communication. Baltimore, Md. 7/24/2020. 3. Manabe, R., Kovalenko, M., Webb, D. J., Horwitz, A. R. GIT1 functions in a motile, multi-molecular signaling complex that regulates protrusive activity and cell migration. J. Cell Sci. 115: 1497-1510, 2002.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu GIT1

Target: GIT1

Andere Bezeichnung: GIT1

Hintergrund:

Synonyms: DNA repair protein XRCC2, X-ray repair cross-complementing protein 2, Xrcc2

Tissue Specificity: Expressed at low level in somatic tissues and at high level in testis.

Background: ARF GTPase-activating protein GIT1 is an enzyme that in humans is encoded by the GIT1 gene. G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration. GIT-1 has also been implicated in neuronal functions including synapse formation and the pathology of Huntington disease. Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains. Htt interacts ly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology.

Molekulargewicht: 84 kDa

Gen-ID: 28964

UniProt: Q9Y2X7