CAPNL1 Antikörper (AA 396-555)

Produktnummer ABIN7601599

Der Maus Monoklonal Anti-CAPNL1-Antikörper wurde für WB und FACS validiert. Er ist geeignet, CAPNL1 in Proben von Human zu detektieren.

Kurzübersicht für CAPNL1 Antikörper (AA 396-555) (ABIN7601599)

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CAPNL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Klon: 2E3

Produktdetails anti-CAPNL1 Antikörper

Bindungsspezifität: AA 396-555

Verwendungszweck: Anti-Calpain 1 Antibody Picoband® (monoclonal, 2E3)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Calpain 1 Antibody Picoband® (monoclonal, 2E3) (ABIN7601599). Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Calpain 1 recombinant protein (Position: Q396-A555). Human Calpain 1 shares 86% amino acid (aa) sequence identity with both mouse and rat Calpain 1.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Smith IJ, et al. Calpain activity is increased in skeletal muscle from gastric cancer patients with no or minimal weight loss. Muscle Nerve, 2011 Mar. 2. Wang JC, et al. Proteolysis by endogenous calpain I leads to the activation of calcineurin in human heart. Clin Lab, 2012.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu CAPNL1

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Andere Bezeichnung: CAPN1

Hintergrund:

Synonyms: Structural maintenance of chromosomes protein 3, SMC protein 3, SMC-3, Basement membrane-associated chondroitin proteoglycan, Bamacan, Chondroitin sulfate proteoglycan 6, Chromosome-associated polypeptide, hCAP, SMC3, BAM, BMH, CSPG6, SMC3L1

Tissue Specificity: Ubiquitous. Expressed in the rod and cone photoreceptors, extending from the tips of the outer segment (OS) through the inner segment (IS) and outer nuclear layer (ONL) and into the synaptic terminals of the outer plexiform layer (ONL). Also detected in the bipolar, horizontal and amacrine cells in the inner nuclear layer (INL), extending to the inner plexiform layer (IPL) and though the ganglion cell layer (GCL) and into the nerve fiber layer (NFL) (at protein level).

Background: CAPN1 is also known as CANP or muCL. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.

Molekulargewicht: 82 kDa

Gen-ID: 823

UniProt: P07384

Pathways: Apoptose