EIF4A1 Antikörper (AA 32-406)

Produktnummer ABIN7601309

Dieses Anti-EIF4A1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von EIF4A1 in WB, IF, IHC, ELISA, ICC und FACS. Geeignet für Human, Ratte und Maus.

Kurzübersicht für EIF4A1 Antikörper (AA 32-406) (ABIN7601309)

Target: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF4A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-EIF4A1 Antikörper

Bindungsspezifität: AA 32-406

Verwendungszweck: Anti-EIF4A1/2/3 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-EIF4A1/2/3 Antibody Picoband® (ABIN7601309). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human EIF4A1/2/3 recombinant protein (Position: D32-I406 ).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human,Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Garcia-Garcia, C., Frieda, K. L., Feoktistova, K., Fraser, C. S., Block, S. M. Factor-dependent processivity in human eIF4A DEAD-box helicase. Science 348: 1486-1488, 2015. 2. Jones, E., Quinn, C. M., See, C. G., Montgomery, D. S., Ford, M. J., Kolble, K., Gordon, S., Greaves, D. R. The linked human elongation initiation factor 4A1 (EIF4A1) and CD68 genes map to chromosome 17p13. Genomics 53: 248-250, 1998. 3. Kim, N.-S., Kato, T., Abe, N., Kato, S. Nucleotide sequence of human cDNA encoding eukaryotic initiation factor 4A1. Nucleic Acids Res. 21: 2012 only, 1993.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu EIF4A1

Target: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Andere Bezeichnung: EIF4A1

Hintergrund:

Synonyms: Eukaryotic initiation factor 4A-I, eIF-4A-I, eIF4A-I, ATP-dependent RNA helicase eIF4A-1, EIF4A1, DDX2A, EIF4A

Tissue Specificity: Adipocytes.

Background: Eukaryotic initiation factor 4A-I is a protein that in humans is encoded by the EIF4A1 gene. It is mapped to 17p13.1. EIF4A1 has been shown to interact with EIF4E and eukaryotic translation initiation factor 4 gamma.

Molekulargewicht: 46 kDa

Gen-ID: 1973

UniProt: P60842