EIF4A1 Antikörper

Produktnummer ABIN7203768

Der Maus Monoklonal anti-EIF4A1 Antikörper wird verwendet zum Nachweis von EIF4A1 in Proben von Human, Ratte und Maus. Er wurde validiert für WB, IF und IHC.

Kurzübersicht für EIF4A1 Antikörper (ABIN7203768)

Target: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EIF4A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Klon: 4A1

Produktdetails anti-EIF4A1 Antikörper

Verwendungszweck: Mouse Anti-eIF4A1 Monoclonal Antibody

Spezifität: The antibody detects endogenous eIF4A1 protein.

Aufreinigung: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: Synthetic Peptide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000-3000,IF 1:100-200,IHC 1:50-300

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.5 % BSA, 0.02 % sodium azide as Preservative and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu EIF4A1

Target: EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))

Andere Bezeichnung: eIF4A1

Hintergrund: EIF4A1 is a 406 amino acid ATP-dependent RNA helicase belonging to the DEAD box helicase family and eukaryotic initiation factor-4A subfamily with a helicase ATP-binding domain and a helicase C-terminal domain. eIF4F eukaryotic translation initiation complex is formed by the association of eIF4A1 with eIF4E and eIF4E kinase (MNK1), eIF4B, eIF4G, PABPC1. It is closely related to eIF4A2. eIF4A1 is a eukaryotic translation initiation factor involved in protein translation/synthesis where it helps in unwinding the secondary structure of RNA in the 5'-UTR of mRNAs, cap recognition, and is necessary for efficient binding mRNA to the 40S small ribosomal subunit, as well as subsequent scanning for the initiator codon. Ubiquitous expression of the enzyme eIF4A1 is seen in most tissues.

Molekulargewicht: 48kD

Gen-ID: 1973

UniProt: P60842