TMPRSS3 Antikörper (AA 3-454)

Produktnummer ABIN7601198

Dieses Anti-TMPRSS3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TMPRSS3 in WB, ELISA, IHC und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für TMPRSS3 Antikörper (AA 3-454) (ABIN7601198)

Target: TMPRSS3 (Transmembrane Protease, Serine 3 (TMPRSS3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TMPRSS3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-TMPRSS3 Antikörper

Bindungsspezifität: AA 3-454

Verwendungszweck: Anti-TMPRSS3 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-TMPRSS3 Antibody (ABIN7601198). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human TMPRSS3 recombinant protein (Position: E3-T454).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ben-Yosef, T., Wattenhofer, M., Riazuddin, S., Ahmed, Z. M., Scott, H. S., Kudoh, J., Shibuya, K., Antonarakis, S. E., Bonne-Tamir, B., Radhakrishna, U., Naz, S., Ahmed, Z., Riazuddin, S., Pandya, A., Nance, W. E., Wilcox, E. R., Friedman, T. B., Morell, R. J. Novel mutations of TMPRSS3 in four DFNB8/B10 families segregating congenital autosomal recessive deafness. (Letter) J. Med. Genet. 38: 396-400, 2001. 2. Fasquelle, L., Scott, H. S., Lenoir, M., Wang, J., Rebillard, G., Goboyard, S., Venteo, S., Francois, F., Mausset-Bonnefont, A.-L., Antonarakis, S. E., Neidhart, E., Chabbert, C., Puel, J.-L., Guipponi, M., Delprat, B. Tmprss3, a transmembrane serine protease deficient in human DFNB8/10 deafness, is critical for cochlear hair cell survival at the onset of hearing. J. Biol. Chem. 286: 17383-17397, 2011. 3. Guipponi, M., Vuagniaux, G., Wattenhofer, M., Shibuya, K., Vazquez, M., Dougherty, L., Scamuffa, N., Guida, E., Okui, M., Rossier, C., Hancock, M., Buchet, K., Reymond, A., Hummler, E., Marzella, P. L., Kudoh, J., Shimizu, N., Scott, H. S., Antonarakis, S. E., Rossier, B. C. The transmembrane serine protease (TMPRSS3) mutated in deafness DFNB8/10 activates the epithelial sodium channel (ENaC) in vitro. Hum. Molec. Genet. 11: 2829-2836, 2002.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu TMPRSS3

Target: TMPRSS3 (Transmembrane Protease, Serine 3 (TMPRSS3))

Andere Bezeichnung: TMPRSS3

Hintergrund:

Synonyms: Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, Erf1, Protein Cl1, TB3-1, ETF1, ERF1, RF1, SUP45L1

Tissue Specificity: Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.

Background: Transmembrane protease, serine 3 is an enzyme that in humans is encoded by the TMPRSS3 gene. This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a serine protease domain, a transmembrane domain, an LDL receptor-like domain, and a scavenger receptor cysteine-rich domain. Serine proteases are known to be involved in a variety of biological processes, whose malfunction often leads to human diseases and disorders. This gene was identified by its association with both congenital and childhood onset autosomal recessive deafness. This gene is expressed in fetal cochlea and many other tissues, and is thought to be involved in the development and maintenance of the inner ear or the contents of the perilymph and endolymph. This gene was also identified as a tumor-associated gene that is overexpressed in ovarian tumors. Alternatively spliced transcript variants have been described.

Molekulargewicht: 48 kDa

Gen-ID: 64699

UniProt: P57727

Pathways: Sensory Perception of Sound