RAD21 Antikörper (AA 292-529)

Produktnummer ABIN7601152

Der Kaninchen Polyklonal Anti-RAD21-Antikörper wurde für WB, ELISA, IF, FACS und ICC validiert. Er ist geeignet, RAD21 in Proben von Human, Maus, Ratte und Affe zu detektieren.

Kurzübersicht für RAD21 Antikörper (AA 292-529) (ABIN7601152)

Target: RAD21 (RAD21 Homolog (RAD21))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAD21 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-RAD21 Antikörper

Bindungsspezifität: AA 292-529

Verwendungszweck: Anti-RAD21 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-RAD21 Antibody Picoband® (ABIN7601152). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human RAD21 recombinant protein (Position: D292-K529).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat, Monkey
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bonora, E., Bianco, F., Cordeddu, L., Bamshad, M., Francescatto, L., Dowless, D., Stanghellini, V. Cogliandro, R. F., Lindberg, G., Mungan, Z., Cefle, K., Ozcelik, T., and 14 others. Mutations in RAD21 disrupt regulation of APOB in patients with chronic intestinal pseudo-obstruction. Gastroenterology 148: 771-782, 2015. 2. Boyle, M. I., Jespersgaard, C., Nazaryan, L., Bisgaard, A.-M., Tumer, Z. A novel RAD21 variant associated with intrafamilial phenotypic variation in Cornelia de Lange syndrome--review of the literature. (Letter) Clin. Genet. 91: 647-649, 2017. 3. Deardorff, M. A., Wilde, J. J., Albrecht, M., Dickinson, E., Tennstedt, S., Braunholz, D., Monnich, M., Yan, Y., Xu, W., Gil-Rodriguez, M. C., Clark, D., Hakonarson, H., and 15 others. RAD21 mutations cause a human cohesinopathy. Am. J. Hum. Genet. 90: 1014-1027, 2012.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu RAD21

Target: RAD21 (RAD21 Homolog (RAD21))

Andere Bezeichnung: RAD21

Hintergrund:

Synonyms: Tubulin beta chain, Tubulin beta-5 chain, TUBB, TUBB5, OK/SW-cl.56

Tissue Specificity: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Background: Double-strand-break repair protein rad21 homolog is a protein that in humans is encoded by the RAD21 gene. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

Molekulargewicht: 72 kDa

Gen-ID: 5885

UniProt: O60216

Pathways: Positive Regulation of Endopeptidase Activity