SH3KBP1 Antikörper (AA 256-629)

Produktnummer ABIN7600938

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SH3KBP1 in WB, ELISA, ICC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für SH3KBP1 Antikörper (AA 256-629) (ABIN7600938)

Target: SH3KBP1 (SH3-Domain Kinase Binding Protein 1 (SH3KBP1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SH3KBP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-SH3KBP1 Antikörper

Bindungsspezifität: AA 256-629

Verwendungszweck: Anti-SH3KBP1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-SH3KBP1 Antibody Picoband® (ABIN7600938). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human SH3KBP1 recombinant protein (Position: D256-Q629).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Feng, L., Wang, J.-T., Jin, H., Qian, K., Geng, J.-G. SH3KBP1-binding protein 1 prevents epidermal growth factor receptor degradation by the interruption of c-Cbl-CIN85 complex. Cell Biochem. Funct. 29: 589-596, 2011. 2. Keller, B., Shoukier, M., Schulz, K., Bhatt, A., Heine, I., Strohmeier, V., Speckmann, C., Engels, N., Warnatz, K., Wienands, J. Germline deletion of CIN85 in humans with X chromosome-linked antibody deficiency. J. Exp. Med. 215: 1327-1336, 2018. 3. Kometani, K., Yamada, T., Sasaki, Y., Yokosuka, T., Saito, T., Rajewsky, K., Ishiai, M., Hikida, M., Kurosaki, T. CIN85 drives B cell responses by linking BCR signals to the canonical NF-kappaB pathway. J. Exp. Med. 208: 1447-1457, 2011.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu SH3KBP1

Target: SH3KBP1 (SH3-Domain Kinase Binding Protein 1 (SH3KBP1))

Andere Bezeichnung: SH3KBP1

Hintergrund:

Synonyms: Elongator complex protein 3, Help3, ELP3

Tissue Specificity: Expressed at low levels in the more differentiated suprabasal regions of the small intestine, and at higher levels in the colon, mainly in the upper region and in scattered cells throughout the remaining epithelium. Also expressed in epithelial cells of bladder, ileum and stomach and at lower levels in pancreas and earskin. The phosphorylated form is nearly exclusively expressed in goblet cells of the small intestine and in the lumen-proximal cells of the colon (at protein level). Also expressed in jejunum and duodenum.

Background: SH3 domain-containing kinase-binding protein 1 is an adaptor protein that in humans is encoded by the SH3KBP1 gene. This gene encodes an adapter protein that contains one or more N-terminal Src homology domains, a proline rich region and a C-terminal coiled-coil domain. The encoded protein facilitates protein-protein interactions and has been implicated in numerous cellular processes including apoptosis, cytoskeletal rearrangement, cell adhesion and in the regulation of clathrin-dependent endocytosis. Alternate splicing results in multiple transcript variants encoding distinct isoforms.

Molekulargewicht: 85 kDa

Gen-ID: 30011

UniProt: Q96B97

Pathways: EGFR Signaling Pathway, EGFR Downregulation