Osteoactivin Antikörper (AA 164-564)

Produktnummer ABIN7600217

Dieses Anti-Osteoactivin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Osteoactivin in WB, ELISA, IHC, FACS, ICC und IF. Geeignet für Human, Maus und Ratte.

Kurzübersicht für Osteoactivin Antikörper (AA 164-564) (ABIN7600217)

Target: Osteoactivin (GPNMB)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Osteoactivin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-Osteoactivin Antikörper

Bindungsspezifität: AA 164-564

Verwendungszweck: Anti-GPNMB/Gpnmb Picoband® Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-GPNMB/Gpnmb Picoband® Antibody (ABIN7600217). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse GPNMB/Gpnmb recombinant protein (Position: R164-D564).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Lu, H., Wang, X., Pullen, M., Guan, H., Chen, H., Sahu, S., Zhang, B., Chen, H., Williams, R. W., Geisert, E. E., Lu, L., Jablonski, M. M. Genetic dissection of the Gpnmb network in the eye. Invest. Ophthal. Vis. Sci. 52: 4132-4142, 2011. 2. Weterman, M. A. J., Ajubi, N., van Dinter, I. M. R., Degen, W. G. J., van Muijen, G. N. P., Ruiter, D. J., Bloemers, H. P. J. Nmb, a novel gene, is expressed in low-metastatic human melanoma cell lines and xenografts. Int. J. Cancer 60: 73-81, 1995. 3. Yang, C.-F., Lin, S.-P., Chiang, C.-P., Wu, Y.-H., H'ng, W. S., Chang, C.-P., Chen, Y.-T., Wu, J.-Y. Loss of GPNMB causes autosomal-recessive amyloidosis cutis dyschromica in humans. Am. J. Hum. Genet. 102: 219-232, 2018.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Osteoactivin

Target: Osteoactivin (GPNMB)

Andere Bezeichnung: Gpnmb

Hintergrund:

Synonyms: Transmembrane glycoprotein NMB, DC-HIL, Dendritic cell-associated transmembrane protein, Osteoactivin, Gpnmb, Dchil, Hgfin,Nmb

Tissue Specificity: Monocyte/macrophage specific.

Background: Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. It is mapped to 6 B2.3, 6 23.82 cM. The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 64 kDa

Gen-ID: 93695

UniProt: Q99P91