AIM2 Antikörper (AA 136-354)

Produktnummer ABIN7599984

Der Kaninchen Polyklonal Anti-AIM2-Antikörper wurde für WB, ELISA und FACS validiert. Er ist geeignet, AIM2 in Proben von Maus zu detektieren.

Kurzübersicht für AIM2 Antikörper (AA 136-354) (ABIN7599984)

Target: AIM2 (Absent in Melanoma 2 (AIM2))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AIM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-AIM2 Antikörper

Bindungsspezifität: AA 136-354

Verwendungszweck: Anti-Aim2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Aim2 Antibody Picoband® (ABIN7599984). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Aim2 recombinant protein (Position: Q136-E354).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. DeYoung KL, Ray ME, Su YA, Anzick SL, Johnstone RW, Trapani JA, Meltzer PS, Trent JM (Aug 1997). "Cloning a novel member of the human interferon-inducible gene family associated with control of tumorigenicity in a model of human melanoma". Oncogene 15 (4): 453-7. 2. "Entrez Gene: AIM2 absent in melanoma 2". 3. Woerner SM, Kloor M, Schwitalle Y, Youmans H, von Knebel Doeberitz M, Gebert J, Dihlmann S (2007). "The putative tumor suppressor AIM2 is frequently affected by different genetic alterations in microsatellite unstable colon cancers". Genes Chromosomes Cancer 46 (12): 1080-9.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu AIM2

Target: AIM2 (Absent in Melanoma 2 (AIM2))

Andere Bezeichnung: Aim2

Hintergrund:

Synonyms: Coagulation factor V, Activated protein C cofactor, Proaccelerin, labile factor, Coagulation factor V heavy chain, Coagulation factor V light chain, F5

Tissue Specificity: Plasma.

Background: Interferon-inducible protein AIM2,also known asabsent in melanoma 2or simplyAIM2,is aproteinthat in humans is encoded by theAIM2gene. It is mapped to 1q22. AIM2 is a member of theIfi202/IFI16family. It plays a putative role in tumorigenic reversion and may control cell proliferation.Interferon-gammainduces expression of AIM2. Though there has been virtually no biochemistry performed, a model based on cell-based or in vivo experiments has led to the current model of how AIM2 triggers the inflammasome. The C-terminal HIN domain binds double stranded DNA(either viral, bacterial, or even host) and acts as a cytosolic dsDNA sensor. This leads to the oligomerization of the inflammasome complex. The N-terminal pyrin domain of AIM2 interacts with the pyrin domain of another proteinASC(or Apoptosis-associated Speck-like protein containing a caspase activation and recruitment domain). ASC also contains aCARD domain(caspase activation and recruitment domain), that recruits procaspase-1 to the complex. This leads to the autoactivation ofcaspase-1, an enzyme that processes proinflammatorycytokines(IL-1bandIL-18).

Molekulargewicht: 39 kDa

Gen-ID: 383619

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome