UBE2N Antikörper (AA 13-152)

Produktnummer ABIN7599924

Der Kaninchen Polyklonal Anti-UBE2N-Antikörper wurde für WB, ELISA, IF, ICC und FACS validiert. Er ist geeignet, UBE2N in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für UBE2N Antikörper (AA 13-152) (ABIN7599924)

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UBE2N Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-UBE2N Antikörper

Bindungsspezifität: AA 13-152

Verwendungszweck: Anti-Ubc13/UBE2N Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Ubc13/UBE2N Antibody Picoband® (ABIN7599924). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Ubc13/UBE2N recombinant protein (Position: Q13-I152).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen, J., Hao, L., Li, C., Ye, B., Du, Y., Zhang, H., Long, B., Zhu, P., Liu, B., Yang, L., Li, P., Tian, Y., Fan, Z. The endoplasmic reticulum adaptor protein ERAdP initiates NK cell activation via the Ubc13-mediated NF-kappa-B pathway. J. Immun. 194: 1292-303, 2015. 2. Fukushima, T., Matsuzawa, S., Kress, C. L., Bruey, J. M., Krajewska, M., Lefebvre, S., Zapata, J. M., Ronai, Z., Reed, J. C. Ubiquitin-conjugating enzyme Ubc13 is a critical component of TNF receptor-associated factor (TRAF)-mediated inflammatory responses. Proc. Nat. Acad. Sci. 104: 6371-6376, 2007. 3. Gross, M. B. Personal Communication. Baltimore, Md. 7/25/2014.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu UBE2N

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Andere Bezeichnung: UBE2N

Hintergrund:

Synonyms: Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog, DDX4, VASA

Tissue Specificity: Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.

Background: Ubiquitin-conjugating enzyme E2 N is a protein that in humans is encoded by the UBE2N gene. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.

Molekulargewicht: 17 kDa

Gen-ID: 7334

UniProt: P61088

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Positive Regulation of Response to DNA Damage Stimulus, Ubiquitin Proteasome Pathway