EDEM1 Antikörper (AA 124-657)

Produktnummer ABIN7599876

Der Kaninchen Polyklonal Anti-EDEM1-Antikörper wurde für WB, ELISA und FACS validiert. Er ist geeignet, EDEM1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für EDEM1 Antikörper (AA 124-657) (ABIN7599876)

Target: EDEM1 (ER Degradation Enhancer, Mannosidase alpha-Like 1 (EDEM1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EDEM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-EDEM1 Antikörper

Bindungsspezifität: AA 124-657

Verwendungszweck: Anti-EDEM1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-EDEM1 Antibody Picoband® (ABIN7599876). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human EDEM1 recombinant protein (Position: P124-I657). Human EDEM1 shares 96.6% amino acid (aa) sequence identity with mouse EDEM1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Hosokawa, N., Wada, I., Hasegawa, K., Yorihuzi, T., Tremblay, L. O., Herscovics, A., Nagata, K. A novel ER alpha-mannosidase-like protein accelerates ER-associated degradation. EMBO Rep. 2: 415-422, 2001. 2. Molinari, M., Calanca, V., Galli, C., Lucca, P., Paganetti, P. Role of EDEM in the release of misfolded glycoproteins from the calnexin cycle. Science 299: 1397-1400, 2003. 3. Nagase, T., Seki, N., Ishikawa, K., Ohira, M., Kawarabayasi, Y., Ohara, O., Tanaka, A., Kotani, H., Miyajima, N., Nomura, N. Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain. DNA Res. 3: 321-329, 1996.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu EDEM1

Target: EDEM1 (ER Degradation Enhancer, Mannosidase alpha-Like 1 (EDEM1))

Andere Bezeichnung: EDEM1

Hintergrund:

Synonyms: EDEM1, EDEM, KIAA0212, ER degradation-enhancing alpha-mannosidase-like protein 1

Background: Enables mannosyl-oligosaccharide 1,2-alpha-mannosidase activity and misfolded protein binding activity. Involved in positive regulation of retrograde protein transport, ER to cytosol, protein targeting to ER, and proteolysis involved in protein catabolic process. Located in aggresome and endoplasmic reticulum quality control compartment.

Molekulargewicht: 66 kDa

Gen-ID: 9695

UniProt: Q92611

Pathways: ER-Nucleus Signaling