PAK1 Antikörper (AA 122-243)

Produktnummer ABIN7599852

Der Kaninchen Polyklonal Anti-PAK1-Antikörper wurde für WB, IHC, ELISA, IF, ICC und FACS validiert. Er ist geeignet, PAK1 in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für PAK1 Antikörper (AA 122-243) (ABIN7599852)

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-PAK1 Antikörper

Bindungsspezifität: AA 122-243

Verwendungszweck: Anti-PAK1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-PAK1 Antibody Picoband® (ABIN7599852). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PAK1 recombinant protein (Position: Q122-K243).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Brown JL, Stowers L, Baer M, Trejo J, Coughlin S, Chant J (Mar 1997). "Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway". Curr Biol 6 (5): 598-605. 2. Bekri S, Adelaide J, Merscher S, Grosgeorge J, Caroli-Bosc F, Perucca-Lostanlen D, Kelley PM, Pebusque MJ, Theillet C, Birnbaum D, Gaudray P (Apr 1998). "Detailed map of a region commonly amplified at 11q13-->q14 in human breast carcinoma". Cytogenet Cell Genet 79 (1-2): 125-31. 3. "Entrez Gene: PAK1 p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)". 4. Bekri, S., Adelaide, J., Merscher, S., Grosgeorge, J., Caroli-Bosc, F., Perucca-Lostanlen, D., Kelley, P. M., Pebusque, M.-J., Theillet, C., Birnbaum, D., Gaudray, P. Detailed map of a region commonly amplified at 11q13-q14 in human breast carcinoma. Cytogenet. Cell Genet. 79: 125-131, 1997.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Andere Bezeichnung: PAK1

Hintergrund:

Synonyms: Neutrophil gelatinase-associated lipocalin,NGAL,Lipocalin-2,SV-40-induced 24P3 protein,Siderocalin LCN2,p25,Lcn2,

Tissue Specificity: Detected in lung, spleen, uterus, vagina and epididymis. .

Background: Serine/threonine-protein kinase PAK 1 is an enzyme that in humans is encoded by the PAK1 gene. PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activated kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternative transcripts of this gene have been found, but their full-length natures have not yet been determined. The PAK1 gene is mapped to 11q13-q14 by inclusion within a mapped clone.

Molekulargewicht: 68 kDa

Gen-ID: 5058

UniProt: Q13153

Pathways: MAPK Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis