Dynactin 1 Antikörper (AA 1042-1278)

Produktnummer ABIN7599660

Dieses Anti-Dynactin 1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Dynactin 1 in WB, ELISA, IHC, IF, ICC und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für Dynactin 1 Antikörper (AA 1042-1278) (ABIN7599660)

Target: Dynactin 1 (DCTN1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Dynactin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-Dynactin 1 Antikörper

Bindungsspezifität: AA 1042-1278

Verwendungszweck: Anti-DCTN1/p150-glued Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-DCTN1/p150-glued Antibody Picoband® (ABIN7599660). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human DCTN1/p150-glued recombinant protein (Position: K1042-S1278).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Caroppo, P., Le Ber, I., Clot, F., Rivaud-Pechoux, S., Camuzat, A., De Septenville, A., Boutoleau-Bretonniere, C., Mourlon, V., Sauvee, M., Lebouvier, T., Bonnet, A.-M., Levy, R., Vercelletto, M., Brice, A. DCTN1 mutation analysis in families with progressive supranuclear palsy-like phenotypes. JAMA Neurol. 71: 208-215, 2014. 2. Collin, G. B., Nishina, P. M., Marshall, J. D., Naggert, J. K. Human DCTN1: genomic structure and evaluation as a candidate for Alstrom syndrome. Genomics 53: 359-364, 1998. 3. Eaton, B. A., Fetter, R. D., Davis, G. W. Dynactin is necessary for synapse stabilization. Neuron 34: 729-741, 2002.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Dynactin 1

Target: Dynactin 1 (DCTN1)

Andere Bezeichnung: DCTN1

Hintergrund:

Synonyms: Probetacellulin, Betacellulin, BTC, BTC

Tissue Specificity: Highly expressed in testis.

Background: Dynactin subunit 1 is a protein that in humans is encoded by the DCTN1 gene. This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains ly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).

Molekulargewicht: 150 kDa

Gen-ID: 1639

UniProt: Q14203

Pathways: M Phase, ER-Nucleus Signaling