Cyclin B1 Antikörper (AA 1-383)

Produktnummer ABIN7599335

Der Kaninchen Polyklonal Anti-Cyclin B1-Antikörper wurde für WB, IHC, ELISA, IF, FACS und ICC validiert. Er ist geeignet, Cyclin B1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Cyclin B1 Antikörper (AA 1-383) (ABIN7599335)

Target: Cyclin B1 (CCNB1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-Cyclin B1 Antikörper

Bindungsspezifität: AA 1-383

Verwendungszweck: Anti-Cyclin B1/CCNB1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Cyclin B1/CCNB1 Antibody Picoband® (ABIN7599335). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Cyclin B1/CCNB1 recombinant protein (Position: M1-L383).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Matsuo, T., Yamaguchi, S., Mitsui, S., Emi, A., Shimoda, F., Okamura, H. Control mechanism of the circadian clock for timing of cell division in vivo. Science 302: 255-259, 2003. 2. Milatovich, A., Francke, U. Human cyclin B1 gene (CCNB1) assigned to chromosome 5 (q13-qter). Somat. Cell Molec. Genet. 18: 303-307, 1992. 3. Moore, J. D., Kirk, J. A., Hunt, T. Unmasking the S-phase-promoting potential of cyclin B1. Science 300: 987-990, 2003.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Cyclin B1

Target: Cyclin B1 (CCNB1)

Andere Bezeichnung: CCNB1

Hintergrund:

Synonyms: G2/mitotic-specific cyclin-B1, CCNB1, CCNB

Background: G2/mitotic-specific cyclin-B1 is a protein that in humans is encoded by the CCNB1 gene. It is mapped to 5q13.2. The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle.

Molekulargewicht: 55 kDa

Gen-ID: 891

UniProt: P14635

Pathways: Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase