NHEJ1 Antikörper (AA 1-273)

Produktnummer ABIN7599198

Dieses Anti-NHEJ1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von NHEJ1 in WB, ELISA, IF, IHC, FACS und ICC. Geeignet für Human und Maus.

Kurzübersicht für NHEJ1 Antikörper (AA 1-273) (ABIN7599198)

Target: NHEJ1 (Nonhomologous End-Joining Factor 1 (NHEJ1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NHEJ1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-NHEJ1 Antikörper

Bindungsspezifität: AA 1-273

Verwendungszweck: Anti-XLF/NHEJ1 Picoband® Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-XLF/NHEJ1 Picoband® Antibody (ABIN7599198). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human XLF/NHEJ1 recombinant protein (Position: M1-E273).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Callebaut, I., Malivert, L., Fischer, A., Mornon, J.-P., Revy, P., de Villartay, J.-P. Cernunnos interacts with the XRCC4-DNA-ligase IV complex and is homologous to the yeast nonhomologous end-joining factor Nej1. J. Biol. Chem. 281: 13857-13860, 2006. 2. Cantagrel, V., Lossi, A.-M., Lisgo, S., Missirian, C., Borges, A., Philip, N., Fernandez, C., Cardoso, C., Figarella-Branger, D., Moncla, A., Lindsay, S., Dobyns, W. B., Villard, L. Truncation of NHEJ1 in a patient with polymicrogyria. Hum. Mutat. 28: 356-364, 2007. 3. Dai, Y., Kysela, B., Hanakahi, L. A., Manolis, K., Riballo, E., Stumm, M., Harville, T. O., West, S. C., Oettinger, M. A., Jeggo, P. A. Nonhomologous end joining and V(D)J recombination require an additional factor. Proc. Nat. Acad. Sci. 100: 2462-2467, 2003.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu NHEJ1

Target: NHEJ1 (Nonhomologous End-Joining Factor 1 (NHEJ1))

Andere Bezeichnung: NHEJ1

Hintergrund:

Synonyms: Non-homologous end-joining factor 1, Protein cernunnos, XRCC4-like factor, NHEJ1, XLF

Tissue Specificity: Widely expressed.

Background: Non-homologous end-joining factor 1 (NHEJ1), also known as Cernunnos or XRCC4-like factor (XLF), is a protein that in humans is encoded by the NHEJ1 gene. It is mapped to 2q35. Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.

Molekulargewicht: 38 kDa

Gen-ID: 79840

UniProt: Q9H9Q4

Pathways: DNA Reparatur