GRB10 Antikörper (AA 1-251)

Produktnummer ABIN7599161

Der Maus Monoklonal Anti-GRB10-Antikörper wurde für WB und FACS validiert. Er ist geeignet, GRB10 in Proben von Human, Ratte und Affe zu detektieren.

Kurzübersicht für GRB10 Antikörper (AA 1-251) (ABIN7599161)

Target: GRB10 (Growth Factor Receptor-Bound Protein 10 (GRB10))

Reaktivität: Human, Ratte, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GRB10 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Klon: 5H7

Produktdetails anti-GRB10 Antikörper

Bindungsspezifität: AA 1-251

Verwendungszweck: Anti-GRB10 Antibody Picoband® (monoclonal, 5H7)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-GRB10 Antibody Picoband® (monoclonal, 5H7) (ABIN7599161). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human GRB10 recombinant protein (Position: M1-K251).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Monkey, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Arnaud, P., Monk, D., Hitchins, M., Gordon, E., Dean, W., Beechey, C. V., Peters, J., Craigen, W., Preece, M., Stanier, P., Moore, G. E., Kelsey, G.Conserved methylation imprints in the human and mouse GRB10 genes with divergent allelic expression suggests differential reading of the same mark.Hum. Molec. Genet. 12: 1005-1019, 2003. 2. Charalambous, M., Smith, F. M., Bennett, W. R., Crew, T. E., Mackenzie, F., Ward, A.Disruption of the imprinted Grb10 gene leads to disproportionate overgrowth by an Igf2-independent mechanism.Proc. Nat. Acad. Sci. 100: 8292-8297, 2003. 3. Frantz, J. D., Giorgetti-Peraldi, S., Ottinger, E. A., Shoelson, S. E.Human GRB-IR-beta/GRB10: splice variants of an insulin and growth factor receptor-binding protein with PH and SH2 domains.J. Biol. Chem. 272: 2659-2667, 1997.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu GRB10

Target: GRB10 (Growth Factor Receptor-Bound Protein 10 (GRB10))

Andere Bezeichnung: GRB10

Hintergrund:

Synonyms: Deoxycytidine kinase, dCK, DCK

Tissue Specificity: Expressed ubiquitously in normal tissues.

Background: GRB10, Growth factor receptor-bound protein 10, also known as insulin receptor-binding protein Grb-IR is a protein that in humans is encoded by the GRB10 gene. The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors (e.g., IGF1R and IGF2R). Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner. Alternatively spliced transcript variants encoding different isoforms have been identified.

Molekulargewicht: 67 kDa

Gen-ID: 2887

UniProt: Q13322

Pathways: Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2