CRYAA Antikörper (AA 1-173)

Produktnummer ABIN7599030

Dieses Anti-CRYAA-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CRYAA in WB, IF und ICC. Geeignet für Human, Maus und Ratte.

Kurzübersicht für CRYAA Antikörper (AA 1-173) (ABIN7599030)

Target: CRYAA (Crystallin, alpha A (CRYAA))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CRYAA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 10B9

Produktdetails anti-CRYAA Antikörper

Bindungsspezifität: AA 1-173

Verwendungszweck: Anti-Alpha A Crystallin Antibody Picoband® (monoclonal, 10B9)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Alpha A Crystallin Antibody Picoband® (monoclonal, 10B9) (ABIN7599030). Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human Alpha A Crystallin recombinant protein (Position: M1-S173). Human Alpha A Crystallin shares 94.8% amino acid (aa) sequence identity with both mouse and rat Alpha A Crystallin.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. "Entrez Gene: CRYAA crystallin, alpha A". 2. Derham BK, Harding JJ (1999). "Alpha-crystallin as a molecular chaperone.". Progress in retinal and eye research 18 (4): 463-509. 3. Jaworski CJ, Piatigorsky J (1989). "A pseudo-exon in the functional human alpha A-crystallin gene.". Nature337 (6209): 752-4.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu CRYAA

Target: CRYAA (Crystallin, alpha A (CRYAA))

Andere Bezeichnung: CRYAA

Hintergrund:

Synonyms: Ras-related protein Rab-5A, RAB5A, RAB5

Tissue Specificity: Widely expressed in fetal and adult tissues.

Background: Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone, instead they hold them in large soluble aggregates. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed, alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC).

Molekulargewicht: 20-23 kDa

Gen-ID: 1409

UniProt: P02489

Pathways: M Phase