GFP Antikörper

Produktnummer ABIN7595339

Dieser Carp Polyklonal Antikörper erkennt spezifisch GFP in WB, ELISA, IHC und FACS. Er zeigt eine Reaktivität gegenüber Aequorea victoria.

Kurzübersicht für GFP Antikörper (ABIN7595339)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Carp

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-GFP Antikörper

Spezifität: Specific for EGFP/GFP/Venus

Kreuzreaktivität (Details): Any organism expressing EGFP/GFP/Venus.

Aufreinigung: IgM (mainly)

Immunogen: Recombinant EGFP/GFP/Venus protein was used as the antigen.

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: IHC WB ELISA FC. A working concentration of 5-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Use Rabbit anti Carp IgM (H+L) adaptobody at 1:1000 dilution to be able to use your usual anti rabbit secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term.

Haltbarkeit: 12 months

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: EGFP/GFP/Venus

Hintergrund: GFP is a naturally fluorescent protein discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of aequorin's blue light blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488; Emission max (nm): 509; Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer reporter of gene expression or as a measure of protein-protein interactions.