GFP Antikörper

Produktnummer ABIN7595317

Dieses Anti-GFP-Antikörper ist ein Huhn Polyklonal-Antikörper zur Detektion von GFP in WB und IHC. Geeignet für Aequorea victoria.

Kurzübersicht für GFP Antikörper (ABIN7595317)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-GFP Antikörper

Verwendungszweck: Chicken antibody to EGFP/GFP/Venus

Spezifität: Specific for EGFP/GFP/Venus

Kreuzreaktivität (Details): Any organism expressing EGFP/GFP/Venus.

Aufreinigung: IgY purified on IgY column and by ammonium sulfate cut

Immunogen: Recombinant EGFP/GFP/Venus protein was used as the antigen.

Isotyp: IgY

Anwendungsinformationen

Applikationshinweise: IHC WB. A dilution of 1 : 1000 is recommended. The optimal dilution should be determined by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in 100 µL of DI sterile water. Centrifuge to remove any insoluble material.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Haltbarkeit: 12 months

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: EGFP/GFP/Venus

Hintergrund: GFP is a naturally fluorescent protein discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of aequorin's blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488, Emission max (nm): 509, Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer reporter of gene expression or as a measure of protein-protein interactions.