MRGPRD Antikörper (Intracellular)

Produktnummer ABIN7581915

Dieses Anti-MRGPRD-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MRGPRD in WB, IHC und IF. Geeignet für Ratte.

Kurzübersicht für MRGPRD Antikörper (Intracellular) (ABIN7581915)

Target: MRGPRD (MAS-Related GPR, Member D (MRGPRD))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MRGPRD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: KO Validated

Produktdetails anti-MRGPRD Antikörper

Bindungsspezifität: AA 285-299, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to MRGPRD (GPCR TGR7) Receptor

Sequenz: (C)KSHRLQESLG AVLGR

Spezifität: Intracellular, C-terminus

Kreuzreaktivität (Details): Not recommended for human samples.

Homologie: Mouse - identical It will not recognize MRGPRD from human samples

Produktmerkmale: Anti-MRGPRD (GPCR TGR7) Antibody (ABIN7581915) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MRGPRD from rat and mouse samples. The antibody will not recognize MRGPRD from human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)KSHRLQESLGAVLGR, corresponding to amino acid residues 285-299 of rat MRGPRD

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:400

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu MRGPRD

Target: MRGPRD (MAS-Related GPR, Member D (MRGPRD))

Andere Bezeichnung: MRGPRD

Hintergrund: Mrgd, Mas-related G-protein coupled receptor member D, G-protein coupled receptor TGR7, β-Alanine receptor,Mas-related G-protein coupled receptor member D (MRGPRD) is expressed in the dorsal root ganglia, specifically in non-peptidergic, small-diameter, IB4+ C-fiber neurons that innervate the outer layers of the mammalian skin. They transmit polymodal nociceptive information from the skin epidermis to the substantia gelatinosa of the spinal cord.MRGPRD receptor expressing neurons are essential for the full expression of mechanical nociception. Thus, MRGPRD represents an attractive therapeutic target for the development of agents for treating pain1,2.The Mrg family in mice is comprised of six single-copy genes: MrgD, MrgE, MrgF/RTA, MrgG, MrgH/GPR90, and MAS1. In addition, there are three large subfamilies: MrgA, MrgB,and MrgC3.MRGPRD receptor is activated by β-alanine and is able to inhibit KCNQ/M-type potassium channels and to increase excitability of sensory neurons4,5. MRGPRD has the ability to form heterodimers and is encoded by the TGR7 gene. This receptor is co-expressed with two nociceptors, including purinergic receptor (P2X3) and a vanilloid receptor (VR1)5.

Gen-ID: 293648

UniProt: Q7TN41