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MRGPRD Antikörper (Cytoplasmic Domain)

MRGPRD Reaktivität: Human IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1049069
$500.50
Zzgl. Versandkosten $45.00
50 μg
Lieferung in 11 bis 14 Werktagen
  • Target Alle MRGPRD Antikörper anzeigen
    MRGPRD (MAS-Related GPR, Member D (MRGPRD))
    Bindungsspezifität
    • 15
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reaktivität
    • 24
    • 2
    • 2
    • 1
    Human
    Wirt
    • 26
    • 1
    Kaninchen
    Klonalität
    • 27
    Polyklonal
    Konjugat
    • 11
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser MRGPRD Antikörper ist unkonjugiert
    Applikation
    • 13
    • 13
    • 8
    • 7
    • 7
    • 3
    • 3
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human MRGPRD. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human (100%) Gorilla (94%) Gibbon (89%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 18 amino acid peptide from 4th cytoplasmic domain of human MRGPRD. Percent identity with other species by BLAST analysis: Human (100%), Gorilla (94%), Gibbon (89%).
  • Applikationshinweise
    Approved: IHC, IHC-P (10 μg/mL)
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    MRGPRD (MAS-Related GPR, Member D (MRGPRD))
    Andere Bezeichnung
    MRGD / MRGPRD (MRGPRD Produkte)
    Synonyme
    MRGD, TGR7, Gm499, MrgD, Mrgd, MAS related GPR family member D, mas-related G-protein coupled receptor member D, MAS-related GPR, member D, MRGPRD, LOC615853, Mrgprd
    Hintergrund
    Name/Gene ID: MRGPRD
    Subfamily: Orphan-A
    Family: GPCR

    Synonyms: MRGPRD, Beta-alanine receptor, MRGD, MAS-related GPR, member D, TGR7
    Gen-ID
    116512
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