PKM Antikörper

Produktnummer ABIN7565975

Der Kaninchen Polyklonal Anti-PKM-Antikörper wurde für ELISA und WB validiert. Er ist geeignet, PKM in Proben von Human zu detektieren.

Kurzübersicht für PKM Antikörper (ABIN7565975)

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKM Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Produktdetails anti-PKM Antikörper

Verwendungszweck: PKM2 Antibody

Kreuzreaktivität (Details): Anti-PKM2 is directed against human PKM2 at an internal position.

Produktmerkmale: Synonyms: rabbit anti-PKM2 antibody, PKM 2, PKM-2, Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor M2-PK, p58, OIP3, OIP-3, PK2, PK3

Aufreinigung: This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Affinity purified Anti-PKM2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human PKM2 protein.

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise: Application Note: This affinity purified antibody has been tested for use in western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band 56-58 kDa in size corresponding to PKM2 by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 1:50,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu PKM

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Andere Bezeichnung: PKM

Hintergrund: Background: Anti-PKM2 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Pyruvate kinase has been shown to be expressed in tumor, embryonic and normal proliferating cells in varying levels. Studies show that when PKM2 is secreted it can induce EGFR phosphorylation which stimulates the EGFR downstream pathway. Previously PKM has been shown to be critical for aerobic glycolysis. Four isoforms of PKM play alternative roles within the system and post-translational modifications along these isoforms induce multiple pathways making this Anti-PKM2 antibody ideal for investigators involved in cancer research and post-translational modifications.

Gen-ID: 5315

NCBI Accession: NP_001193725

UniProt: P14618

Pathways: Warburg Effekt