TET3 Antikörper (N-Term)

Produktnummer ABIN7565849

Der Kaninchen Polyklonal Anti-TET3-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, TET3 in Proben von Human zu detektieren.

Kurzübersicht für TET3 Antikörper (N-Term) (ABIN7565849)

Target: TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TET3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-TET3 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: TET3 Antibody

Produktmerkmale: Synonyms: rabbit anti-TET3 Antibody, TET antibodies, Anti-TET Methylcytosine Dioxygenase 3, Anti-TET3 polyclonal antibody, KIAA0401 antibody, dioxygenase antibodies

Aufreinigung: Anti-TET3 Antibody was prepared from whole rabbit antiserum by delipidation and defibrination. The antiserum was further cross-absorbed against MBP by chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: TET3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a human TET-3 domain containing the N-terminal 160 amino acids of the protein.

Immunogen Type: Recombinant Protein

Anwendungsinformationen

Applikationshinweise: Application Note: Tet3 antibody has been tested by Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect band at ~180 kDa. This antibody is suitable for use by ELISA. Western Blot Dilution: 1:5000 ELISA Dilution: 1:50,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 70 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu TET3

Target: TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))

Andere Bezeichnung: TET3

Hintergrund: Background: Anti-Tet3 is expressed in colon, muscle, adrenal gland and peripheral blood lymphocytes. Anti-Tet3 is involved in the epigenetic chromatin reprogramming of a zygote after its fertilization, and the demethylation occurs before the first cell division in the paternal pronucleus. Tet 3 antibody cannot bind to the maternal pronucleus chromatin due to DPPA3/PGC7 chromatin presence. Tet 3 functions as the dioxygenase which synthesizes a hydroxyl group to 5-methylcystosine (5mC) to form 5-hydroxymethylcystosine. Tet3 mediates the formation of 5-formylcystosine from 5hmC and subsequently 5fC to 5-carboxylcystosine, and consequently Tet3 plays an active role in catalyzing those conversions which have been suggested as the first step for cytosine DNA de-methylation. Tet 3 also recruits O-GlcNAc transferase OGT to aid H2B GlcNAcylation by OGT. Anti-TET3 antibodies are ideal for researchers interested in Epigenetics, Cancer, Chromatin Research and Histone research.

Gen-ID: 200424

NCBI Accession: NP_659430

UniProt: O43151

Pathways: Warburg Effekt