TET3 Antikörper (AA 1159-1329)

Produktnummer ABIN2668513

Es sind 2+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-TET3 Antikörper wird verwendet zum Nachweis von TET3 in Proben von Maus. Er wurde validiert für WB und ChIP.

Kurzübersicht für TET3 Antikörper (AA 1159-1329) (ABIN2668513)

Target: TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TET3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Produktdetails anti-TET3 Antikörper

Bindungsspezifität: AA 1159-1329

Verwendungszweck: Tet3 antibody (pAb)

Aufreinigung: Unpurified

Immunogen: This antibody was raised against a recombinant protein corresponding to amino acids 1159-1329 of mouse Tet3.

Anwendungsinformationen

Applikationshinweise: WB: 1:1,000 - 1:5,000 dilution

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-TET3 Antikörper (ABIN2668513)

Thienpont, Steinbacher, Zhao, DAnna, Kuchnio, Ploumakis, Ghesquière, Van Dyck, Boeckx, Schoonjans, Hermans, Amant, Kristensen, Koh, Mazzone, Coleman, Carell, Carmeliet, Lambrechts: "Tumour hypoxia causes DNA hypermethylation by reducing TET activity." in: Nature, Vol. 537, Issue 7618, pp. 63-68, (2016) (PubMed).

Otani, Kimura, Sharif, Endo, Mishima, Kawakami, Koseki, Shirakawa, Suetake, Tajima: "Cell cycle-dependent turnover of 5-hydroxymethyl cytosine in mouse embryonic stem cells." in: PLoS ONE, Vol. 8, Issue 12, pp. e82961, (2013) (PubMed).

Details zu TET3

Target: TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))

Andere Bezeichnung: Tet3

Hintergrund: Tet3 (tet methylcytosine dioxygenase 3) catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Conversion into 5hmC initiates a process leading to cytosine demethylation through deamination into 5-hydroxymethyluracil (5hmU) and subsequent replacement by unmethylated cytosine by the base excision repair system. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC.

Molekulargewicht: 200 kDa

Gen-ID: 194388

NCBI Accession: NP_898961

Pathways: Warburg Effekt