STAT5A Antikörper (meArg31)

Produktnummer ABIN7565845

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch STAT5A in WB, ELISA und DB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für STAT5A Antikörper (meArg31) (ABIN7565845)

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser STAT5A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Dot Blot (DB)

Produktdetails anti-STAT5A Antikörper

Bindungsspezifität: meArg31

Verwendungszweck: STAT5 R31-Me1 Antibody

Kreuzreaktivität (Details): This antibody is specific for STAT5 R31/Me1 and does not reacts with other STATs surrounding aa31.

Produktmerkmale: Synonyms: rabbit anti-STAT5 methyl Arg31 antibody, rabbit anti-STAT5 R31me antibody, STAT-5, STAT 5, STAT5A, Signal Transducer and Activator of Transcription 5A antibody, STAT-5A, Transcription factor STAT5A antibody

Aufreinigung: Anti-STAT5 R31/Me1 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: STAT5A [Arg31-Methyl] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Arginine 31 of human STAT.

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-STAT5 [Arg31/Me1] antibody has been tested by Dot Blot and is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~90.6 kDa corresponding to STAT5 protein by Western Blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1:500 ELISA Dilution: 1:5000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.57 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu STAT5A

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Andere Bezeichnung: STAT5A

Hintergrund: Background: Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5a and Stat5b. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus.

UniProt: P42229

Pathways: JAK-STAT Signalweg, RTK Signalweg, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation