PAX6 Antikörper

Produktnummer ABIN7565804

Der Maus Monoklonal Anti-PAX6-Antikörper wurde für WB, ELISA und FM validiert. Er ist geeignet, PAX6 in Proben von Human zu detektieren.

Kurzübersicht für PAX6 Antikörper (ABIN7565804)

Target: PAX6 (Paired Box 6 (PAX6))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PAX6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Klon: 7H10-D3-D4-G5

Produktdetails anti-PAX6 Antikörper

Verwendungszweck: PAX6 Antibody

Kreuzreaktivität (Details): Cross-reactivity with PAX6 from other sources has not been determined.

Produktmerkmale: Synonyms: mouse anti-pax6 antibody, Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, AN2, PAX 6 Antibody

Aufreinigung: Anti-PAX6 was purified from clarified ascites by Protein A chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: PAX6 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to human PAX6 protein.

Immunogen Type: Recombinant Protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-PAX6 antibody has been tested by ELISA and Immunofluorescence and suitable for Western Blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 46.7 kDa in size corresponding to PAX6 by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:75,000 - 1:150,000 IF Microscopy Dilution: 1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu PAX6

Target: PAX6 (Paired Box 6 (PAX6))

Andere Bezeichnung: PAX6

Hintergrund: Background: Paired box protein Pax-6 also known as aniridia type II protein (AN2) or oculorhombin is a transcription factor with key functions in the development of the eye, nose, central nervous system and pancreas. Pax6 further seems to be required for the differentiation of pancreatic islet alpha cells. PAX6 has been shown to bind to a promoter element in the glucagon, insulin and somatostatin promoters and regulates the specification of the ventral neuron subtypes by establishing the correct progenitor domains. Isoform 5a appears to function as a molecular switch that specifies target genes. Anti-PAX6 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.

Gen-ID: 5080

NCBI Accession: NP_000271

UniProt: P26367

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis