Rekombinanter GST Antikörper

Produktnummer ABIN7565769

Dieses Anti-GST-Antikörper ist ein Llama Monoklonal-Antikörper zur Detektion von GST in WB und DB. Geeignet für Schistosoma japonicum.

Kurzübersicht für Rekombinanter GST Antikörper (ABIN7565769)

Target: GST (Glutathione S Transferase (GST))

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: Schistosoma japonicum

Wirt: Llama

Klonalität: Monoklonal

Konjugat: Dieser GST Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Dot Blot (DB)

Klon: GST13

Produktdetails anti-GST Antikörper

Verwendungszweck: Recombinant GST Antibody

Produktmerkmale: Synonyms: recombinant anti-GST antibody, Glutathione-S-Transferase antibody

Aufreinigung: Anti-GST (VHH) Antibody is a recombinant antibody. The clone was isolated from a library prepared from a hyper-immunized llama host and purified by affinity chromatography from bacterial culture.

Sterilität: Sterile filtered

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-GST is a his-tagged monoclonal recombinant antibody designed to detect Glutathione-S-Transferase and its conjugates. This antibody has been tested by western blot and dot blot and is intended for use in immunological assays including western blotting, immunofluorescence and fluorescence activated cell sorting (FACS). Fluorochrome conjugated anti-GST can be used for detection of sensitive non-radioactive in situ hybridization probes. The antibody can be labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection of GST-conjugated molecules by immunoblotting or immunofluorescence. Secondary detection can be achieved using conjugated anti-His tag or anti-VHH antibodies. Optimal titers for applications should be confirmed by the end user. Western Blot Dilution: 1:200 Other: Dot blot: 1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer:

Buffer: 1X PBS, pH 7.4

Stabilizer: 20 % (v/v) Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu GST

Target: GST (Glutathione S Transferase (GST))

Andere Bezeichnung: GST

Hintergrund: Background: Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors.  GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer. A single-domain antibody (sdAb) is a small antibody fragment consisting of the monomeric variable domain derived from camelid heavy chain-only immunoglobulins naturally found in llamas, alpacas and camels. Also known as VHH antibodies, these are the smallest functional antigen-binding fragment that occurs in nature (12 - 14 kDa) and are now being used in biotechnology as a novel antibody scaffold. The small size of the VHH single domain antibody makes it very attractive for use in diagnostic imaging and potentially therapeutic applications.