Rekombinanter K-RAS Antikörper (G12D)

Produktnummer ABIN7543443

Der Kaninchen Chimeric anti-K-RAS Antikörper wird verwendet zum Nachweis von K-RAS in Proben von Human. Er wurde validiert für ELISA.

Kurzübersicht für Rekombinanter K-RAS Antikörper (G12D) (ABIN7543443)

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser K-RAS Antikörper ist unkonjugiert

Applikation: ELISA

Klon: D113

Produktdetails anti-K-RAS Antikörper

Bindungsspezifität: G12D

Verwendungszweck: Anti-KRAS G12D mutant [D113], Rabbit IgG, kappa

Spezifität:

This antibody binds the G12D mutant form of human KRAS protein. This ras protein binds GDP/GTP and possess intrinsic GTPase activity, that results in conversion of nucleotide guanosine triphosphate (GTP) into guanosine diphosphate (GDP). This protein plays an important role in many signal transduction pathways and regulation of cell proliferation. Somatic KRAS mutations are highly observed in leukemias, colorectal cancer, pancreatic cancer and lung cancer. KRAS mutation is G12D which is estimated to be present in up to 37 % pancreatic cancers and over 12 % of colorectal cancers.

Application Notes original Clone: The binding reactivity and epitope characterization of this antibody towards human KRAS G12D mutant was determined using ELISA (PMID: 9440050). This antibody can bind both the peptide 'KLVVVGADGVGK' conjugated BSA as well as KRAS G12D mutant in an ELISA.

Produktmerkmale: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with a synthetic dodecapeptide 'KLVVVGADGVGK'.

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu K-RAS

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Andere Bezeichnung: KRAS

Hintergrund: K-Ras, GTPase Kras, KRAS2, RASK2, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, ras p21 protein, p21 ras, D113-2-1

UniProt: P01116-2