Rekombinanter EPH Receptor A3 Antikörper

Produktnummer ABIN7543438

Dieses Anti-EPH Receptor A3-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von EPH Receptor A3 in WB, ELISA, FACS, IF, IP, in vivo und SPR. Geeignet für Human.

Kurzübersicht für Rekombinanter EPH Receptor A3 Antikörper (ABIN7543438)

Target: EPH Receptor A3 (EPHA3)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EPH Receptor A3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), In vivo Studies (in vivo), Surface Plasmon Resonance (SPR)

Klon: IIIA4

Produktdetails anti-EPH Receptor A3 Antikörper

Verwendungszweck: Anti-EpHA3 [IIIA4], Mouse IgG1, kappa

Spezifität: The antibody binds to the native EphA3 globular ephrin-binding domain. The antibody targets a site closely adjacent to the heterotetramerization site on the N-terminal of EPHA3's extracellular domain adjacent to the ligand-binding site and has a high affinity for EPHA3.

Kreuzreaktivität: Maus

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was prepared by intraperitoneal immunization of a BALB/c mouse with LK63 cells.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: This antibody was used for detection of hek expressed on LK63 cells by flow cytometry. Hek antigen was immunoprecipitated using the antibody from 125I-labeled LK63 cell lysates (Boyd et al., 1992, PMID: 1737782). The specificity of the chimeric version of the antibody was confirmed by ELISA analysis (US8664365). In a LK63 xenograft model, administration of the antibody inhibited the tumor growth, decreasing the spreading from the bone marrow to the spleen and other organs and increases the latency of the disease. Similar effects were observed in LK63 EPHA3 knock down xenografts. Further, antibody treatment of a xenograft model using EPHA3-transfected Reh, showed reduction in the bone marrow engraftment and increases the latency of the disease (Charmsaz et al., 2016, PMID: 27922598). The antibody bound to the native EphA3 globular ephrin-binding domain with subnanomolar affinity (KD ~ 0.5 nM) (Smith et al., 2004, PMID: 14660665). The antibody specifically stained EphA3 in 95 % of human tumor tissues from a broad range of cancer types (Vail et al. 2014, PMID: 25125683). The humanized version of the antibody (KB004), was tested in a multi-center Phase 1/2 trial, in patients with EphA3-positive hematologic neoplasms, including Chronic Myelogenous Leukemia, Acute Myeloid Leukemia, Acute lymphoblastic leukemia, Myelodysplastic Syndrome.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu EPH Receptor A3

Target: EPH Receptor A3 (EPHA3)

Andere Bezeichnung: EpHA3

Hintergrund: Ephrin type-A receptor 3, EPH-like kinase 4, EK4, hEK4, HEK, Human embryo kinase, Tyrosine-protein kinase TYRO4, Tyrosine-protein kinase receptor ETK1, Eph-like tyrosine kinase 1, III.A4

UniProt: P29320

Pathways: RTK Signalweg, Regulation of Cell Size