Rekombinanter MSH2 Antikörper

Produktnummer ABIN7543327

Dieses Anti-MSH2-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von MSH2 in WB, ELISA und IHC. Geeignet für Human.

Kurzübersicht für Rekombinanter MSH2 Antikörper (ABIN7543327)

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser MSH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Klon: 1B11-14-16

Produktdetails anti-MSH2 Antikörper

Verwendungszweck: Anti-MSH2 [1B11-14-16], Rabbit IgG, kappa

Spezifität: This antibody binds an epitope on the human DNA mismatch protein MSH2 between amino acids 919-934 (NSFVNEIISRIKVTT). MSH2 forms a heterodimer with MSH6 to make the human MutSα mismatch repair complex. The DNA mismatch repair system (MMR) is composed of enzyme molecules that specifically repair DNA base mismatches. MSH2 is involved in many different forms of DNA repair, including transcription-coupled repair, homologous recombination, and base excision repair. Studies have shown that mutations and/or loss of function of MMR genes are related to the occurrence of a variety of tumors, such as colorectal cancer, skin cancer, ovarian cancer, and endometrial cancer.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with human MSH2 protein peptide (KLH-NSFVNEIISRIKVTT-Cys) conjugated with KLH in equal volume of Freund's complete adjuvant.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The binding specificity of this antibody for human MSH2 was determined using ELISA. This antibody was capable of detecting MSH2 from HCT8 and Biu-87 cell lysates in a western blot. This antibody was also used in the immunohistochemical analysis of normal tissues like tonsil, appendix tissues and cancerous tissues like colorectal and bladder cancer. This antibody shows similar staining of MSH2 protein compared to the commercially available antibody G219-1129 (CN110218251).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Andere Bezeichnung: MSH2

Hintergrund: HMSH2, DNA mismatch repair protein Msh2, MutS protein homolog 2, CGMCC NO.17405, G219-1129

UniProt: P43246

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response