Rekombinanter CD3 Antikörper

Produktnummer ABIN7543308

Der Kaninchen Chimeric Anti-CD3-Antikörper wurde für FACS und in vitro validiert. Er ist geeignet, CD3 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter CD3 Antikörper (ABIN7543308)

Target: CD3

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), In vitro Assay (in vitro)

Klon: CD3B219

Produktdetails anti-CD3 Antikörper

Verwendungszweck: Anti-CD3 [CD3B219], Rabbit IgG, lambda

Spezifität: The antibody is specific for CD3 epsilon.

Kreuzreaktivität: Affe

Produktmerkmale:

Original Species of Ab: Human

Original Format of Ab: IgG4

Aufreinigung: Protein A affinity purified

Isotyp: IgG lambda

Anwendungsinformationen

Applikationshinweise: The antibody was used to construct different CD33 xCD3 bispecific antibodies (IgG4). In vitro T cell mediated cytotoxicity assays were performed using the bispecific antibody and the cytotoxicity was measured by flow cytometry. Ex vivo cytotoxicity assay showed the paired antibody promoted a dose-dependent reduction of total cytotoxicity that correlated with T cell activation after 48 hours (US20210040209A1). The antibody is considered a high affinity binder (Kd= 8 mM) (US20210214440A1). The CD33xCD3 bispecific antibodies specifically induce cell cytotoxicity of MOLM-13 cells and CD33+ monocytes at 48 hr (US20200048349A1).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format for improved compatibility with existing reagents assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD3

Target: CD3

Andere Bezeichnung: CD3

Hintergrund: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, SP34-2

UniProt: P07766

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway