Rekombinanter TIGIT Antikörper

Produktnummer ABIN7543290

Der Kaninchen Chimeric Anti-TIGIT-Antikörper wurde für ELISA, FACS und BR validiert. Er ist geeignet, TIGIT in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter TIGIT Antikörper (ABIN7543290)

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser TIGIT Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS), Blocking Reagent (BR)

Klon: 4A5

Produktdetails anti-TIGIT Antikörper

Verwendungszweck: Anti-TIGIT [4A5], Rabbit IgG, kappa

Spezifität: This antibody binds the extracellular domain of the human TIGIT (T cell immunoreceptor with Ig and ITIM domains). TIGIT is a type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins. It is an immune receptor present on some T cells and natural killer cells (NK). It binds the poliovirus receptor (PVR) with high affinity which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing 9 week old BALB/c mice with recombinant human TIGIT extracellular region Fc fusion protein in complete Freund's adjuvant.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The binding characterization of this antibody towards human TIGIT was done using ELISA and flow cytometry. This antibody can bind human TIGIT extracellular domain Fc fusion protein in an ELISA and is also capable of recognizing TIGIT expressed on HEK293 cells by flow cytometry. The original IgG1 version of this antibody binds human TIGIT with a binding affinity of Kd=3.732 X 10^(-10) M. This antibody was also reported to successfully block the binding of TIGIT to its ligand CD155. This antibody is capable of binding NK cells in vitro. The anti-cancer activity of this antibody was tested on ovarian cancer cell HO8910, human non-small cell lung cancer cell NCl-H358 and human non-small cell lung cancer cell NCl-H1650 (CN109384846).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu TIGIT

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Andere Bezeichnung: TIGIT

Hintergrund: VSTM3, VSIG9, WUCAM, T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3

UniProt: Q495A1

Pathways: Cancer Immune Checkpoints