TIGIT Antikörper (AA 22-141)

Produktnummer ABIN6939305

Produktdetails anti-TIGIT Antikörper

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Bindungsspezifität: AA 22-141

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TIGIT Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Coating (Coat), Staining Methods (StM)

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 22-141) of human TIGIT protein (exact sequence is proprietary)

Klon: TIGIT-3017

Isotyp: IgG2c kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MOLT-4, Prostate or Colon Carcinoma.

Known Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu TIGIT

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Andere Bezeichnung: TIGIT (TIGIT Produkte)

Synonyme: VSIG9 antikoerper, VSTM3 antikoerper, WUCAM antikoerper, ENSMUSG00000071552 antikoerper, Vstm3 antikoerper, RGD1563191 antikoerper, T-cell immunoreceptor with Ig and ITIM domains antikoerper, T cell immunoreceptor with Ig and ITIM domains antikoerper, TIGIT antikoerper, Tigit antikoerper

Hintergrund: TIGIT is a checkpoint inhibitor which binds with high affinity to the poliovirus receptor (PVR), causing increased IL10 secretion, decreased IL12B secretion. TIGIT binding to PVR also causes the suppression of T cell activation by promoting the generation of mature immuno-regulatory dendritic cells. It is expressed at low levels on natural killer (NK) cells, as well as peripheral memory and regulatory CD4+ T cells. At the protein level, it is upregulated following the activation of these cells. Functionally, TIGIT is similar to CTLA4. The ligands for TIGIT include CD155 (signal abrogation) and CD226 (signal stimulation). It has been demonstrated to be upregulated on T cells in many cancers and is an immuno-oncology target for therapy.

Molekulargewicht: 26kDa

Gen-ID: 201633

Pathways: Cancer Immune Checkpoints