Rekombinanter LDLR Antikörper

Produktnummer ABIN7543251

Der Kaninchen Chimeric Anti-LDLR-Antikörper wurde für WB, FACS, IP, IF und Inh validiert. Er ist geeignet, LDLR in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter LDLR Antikörper (ABIN7543251)

Target: LDLR (Low Density Lipoprotein Receptor (LDLR))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser LDLR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Inhibition (Inh)

Klon: C7

Produktdetails anti-LDLR Antikörper

Verwendungszweck: Anti-LDL Receptor [C7], Rabbit IgG, kappa

Spezifität: The antibody recognizes the ligand binding domain. It binds to the bovine LDL-R, the human LDL-R but it does not cross react with the LDL-Rs of rat, mouse, Chinese hamster, rabbit and dog. The LDL receptor is a cell surface protein that binds LDL, the major cholesterol-transport protein in plasma, and mediates its endocytosis.

Kreuzreaktivität: Rind (Kuh)

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was produced by immunization of BALB/C mice with the extra cellular domain of the bovine LDL-R.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The antibody (IgG2b) was employed for the staining of the LDL receptor in normal fibroblast. The antibody competed with LDL for binding to the LDL receptor of intact fibroblast, complete inhibition was achieved at 37 C but not at 4 C (Beisiegel et al., 1981, PMID: 6271765). The antibody detected LDL receptor from human fibroblasts and bovine adrenal cortex by western blot analysis (Beisiegel et al., 1982, PMID: 6290495). The LDL receptor was immunoprecipitated using this antibody (Davis et al., 1987, PMID: 3494949). This antibody was used for detection of LDL receptor expressed on Chang and CHO cells by flow cytometry (Holst et al., 2001, PMID: 11781697) (Ranheim et al., 2006, PMID: 16740646). Immunofluorescence was performed on liver Chang cells using this antibody, showing that the WT protein was localised on the cell surface whereas the two mutant proteins were localised intracellularly (Holst et al., 2001, PMID: 11781697). The antibody was used to inhibit the binding of LDL to its receptor LDLR in brain capillary endothelial cells. Coincubations of LDL with increasing concentrations of the antibody decreased the rate of passage of the LDL through the monolayer, LDL transcytosis was totally abolished at the concentration of 1 mg/mL of antibody. This confirmed that the LDL receptor is involved receptor-mediated transcytosis (Dehouck et al., 1997, PMID: 9265653).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu LDLR

Target: LDLR (Low Density Lipoprotein Receptor (LDLR))

Andere Bezeichnung: LDL Receptor

Hintergrund: LDLR, Low-density lipoprotein receptor, IgG-C7

UniProt: P01130

Pathways: Hepatitis C, Lipid Metabolism