Rekombinanter FSHR Antikörper

Produktnummer ABIN7543213

Der Maus Monoklonal anti-FSHR Antikörper (Klon 9H11) (ABIN7543213) detektiert spezifisch FSHR in FACS, IF und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für Rekombinanter FSHR Antikörper (ABIN7543213)

Target: FSHR (Follicle Stimulating Hormone Receptor (FSHR))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FSHR Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), ELISA

Klon: 9H11

Produktdetails anti-FSHR Antikörper

Verwendungszweck: Anti-FSH receptor [9H11], Mouse IgG2a, lambda

Spezifität:

This antibody binds human and mice FSH receptor. FSH receptor is a G protein-coupled receptor for follitropin, the follicle-stimulating hormone. Through cAMP production activates the downstream PI3K-AKT and ERK1/ERK2 signaling pathways.

Application Notes original Clone: To test if the antibody dependent cellular cytotoxicity elicited by the mouse version of this antibody translated into an in vivo anti-tumor effect, mice were challenged with IDS -Defl29/Vegf-a-Fshr and treated with the mouse version of this antibody. The antibody was able to delay tumor progression. To further characterize the antibody its ability to bind cells expressing human and mouse FHSR, flow cytometry was performed using the mouse version of this antibody on A20 cells, ID8- Defl29/Vegf-a-Fshr cells and human ovarian cancer cell lines like Caov3, OVCAR3, TOV21 G and PC3 with physiological levels of FSHR. Isotype analysis of the antibody was done using ELISA. Finally immunofluorescence was performed on 293T cells transduced with human or murine FSHR using the mouse version of this antibody (WO2020033797).

Kreuzreaktivität: Maus

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing Balb/c mice with an optimized DNA construct encoding human FSHR.

Isotyp: IgG2a, lambda

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2a

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu FSHR

Target: FSHR (Follicle Stimulating Hormone Receptor (FSHR))

Andere Bezeichnung: FSH receptor

Hintergrund: FSHR, FSH-R, Follicle-stimulating hormone receptor, Follitropin receptor, LGR1

UniProt: P23945

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Platelet-derived growth Factor Receptor Signaling