p130 Antikörper (C-Term)

Produktnummer ABIN7540634

Hersteller Produkt- Nr.: 600-401-mp1

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch p130 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für p130 Antikörper (C-Term) (ABIN7540634)

Target: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser p130 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-p130 Antikörper

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 600-401-mp1

Hersteller: Rockland

Verwendungszweck: p130 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human Retinoblastoma-like protein 2.

Aufreinigung: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-p130 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal portion of human Retinoblastoma-like protein 2 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000 - 1:50,000

Western_Blot_Dilution: 1 μg/mL

Kommentare:

Anti-p130 Antibody has been tested in ELISA and WB.  Expect bands ~128.4 kDa in western blot using appropriate tissues or lysates.  Positive control used: MOLT-4 lysate in western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu p130

Target: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Andere Bezeichnung: RBL2

Hintergrund: Rabbit Anti-p130 Antibody, Rabbit Anti-Retinoblastoma-like protein 2 Antibody, RB Transcriptional Corepressor Like 2, 130 KDa Retinoblastoma-Associated Protein, Retinoblastoma-Related Protein 2, Retinoblastoma-Like Protein 2, Retinoblastoma-Like 2, RBR-2,Retinoblastoma-Associated Protein (130 KDa) is a key regulator of entry into cell division. p130 is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression and controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters and is a potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. It binds to cyclins A and E, as well as, binds to and may be involved in the transforming capacity of the adenovirus E1A protein. p130 may act as a tumor suppressor. Anti-p130 Antibody may be useful for researchers interested in Adipogenesis, Retinoblastoma, and Retinal Cancer.

Gen-ID: 5934

NCBI Accession: NP_001310537

UniProt: Q08999

Pathways: Zellzyklus, Mitotic G1-G1/S Phases