TBK1 Antikörper (C-Term)

Produktnummer ABIN7540615

Hersteller Produkt- Nr.: 600-401-mk6

Dieses Anti-TBK1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TBK1 in WB, ELISA, IHC, IF und FM. Geeignet für Human.

Kurzübersicht für TBK1 Antikörper (C-Term) (ABIN7540615)

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TBK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Produktdetails anti-TBK1 Antikörper

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 600-401-mk6

Hersteller: Rockland

Verwendungszweck: TBK1 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human TBK1.

Aufreinigung: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-TBK1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal portion of human TBK1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 5 μg/mL

Immunohistochemistry_Dilution: 1:100

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-TBK1 Antibody has been tested in ELISA, WB, and IF. Expect a band at ~83.6kDa in western blot using appropriate lysates. Positive control used: TBK1 overexpressed lysate, HEK, or NIH3T3 in WB, HeLa in IF.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu TBK1

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Andere Bezeichnung: TBK1

Hintergrund: Rabbit Anti-TRK1 Antibody, Rabbit Anti-TANK Binding Kinase 1 Antibody, NF-Kappa-B-Activating Kinase, TANK-Binding Kinase 1, NAK, T2K, Serine/Threonine-Protein Kinase TBK1, NF-KB-Activating Kinase, EC 2.7.11.1, FTDALS4, IIAE8,TBK1 (TANK Binding Kinase 1) is a serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, it associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. In order to establish such an antiviral state, TBK1 forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes. Under particular conditions, it functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB, or RELA to translocate NF-Kappa-B to the nucleus. It restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on Ser-177, thus enhancing LC3 binding affinity and antibacterial autophagy. TKB1 phosphorylates the SMCR8 component of the C9orf72-SMCR8 complex, promoting autophagosome maturation. And it phosphorylates and activates AKT1. Seems to play a role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, which leads to a negative impact on insulin sensitivity. Attenuates retroviral budding by phosphorylating the endosomal sorting complex required for transport-I (ESCRT-I) subunit VPS37C. And it phosphorylates Borna disease virus (BDV) P protein. Anti-TBK1 Antibody is useful for researchers interested in Frontotemporal Dementia and/or Amyotrophic Lateral Sclerosis 4, acute Infection-Induced 8 Encephalopathy, Toll-like Receptor Signaling Pathway, TNFR1 Pathway, nucleic acid binding, and protein kinase activity.

Gen-ID: 9123

NCBI Accession: NP_001035887

UniProt: Q9UHD2

Pathways: TLR Signalweg, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interaktom