Caspase 12 Antikörper (Internal Region)

Produktnummer ABIN7540612

Hersteller Produkt- Nr.: 600-401-mj3s

Dieses Anti-Caspase 12-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Caspase 12 in WB, ELISA, IF und FM. Geeignet für Maus.

Kurzübersicht für Caspase 12 Antikörper (Internal Region) (ABIN7540612)

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caspase 12 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Produktdetails anti-Caspase 12 Antikörper

Bindungsspezifität: Internal Region

Hersteller Produkt- Nr.: 600-401-mj3s

Hersteller: Rockland

Verwendungszweck: Caspase 12 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against mouse Caspase 12.

Aufreinigung: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-Caspase 12 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Caspase 12 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000 - 1:50,000

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 5 μg/mL

Kommentare:

Anti-Caspase 12 Antibody has been tested in ELISA, IF, and WB.  Positive control used: NIH/3T3 and PC-12 lysates in Western Blot and NIH/3T3 in Immunofluorescence.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Caspase 12

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Andere Bezeichnung: Casp12

Hintergrund: Rabbit Anti-Caspase 12 Antibody, Caspase 12 pseudogene 1, Casp12, Casp-12, CASP12P1,Casp12 (Caspase 12 (Gene/Pseudogene)) has no protease activity. It may reduce cytokine release in response to bacterial lipopolysaccharide during infections. Caspase 12 reduces activation of NF-kappa-B in response to TNF. Caspases (cysteinyl aspartate proteases) are involved in the signaling pathways of apoptosis, necrosis and inflammation. These enzymes can be divided into initiators and effectors. The initiator isoforms are activated by, and interact with, upstream adaptor molecules. Anti-Caspase 12 Antibody is useful for researchers interested in Amyotrophic lateral sclerosis (ALS), ATP synthesis, and Apoptosis research.

Gen-ID: 12364

NCBI Accession: NP_033938

UniProt: O08736

Pathways: Apoptose, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response