ERN1 Antikörper (Internal Region)

Produktnummer ABIN7540596

Hersteller Produkt- Nr.: 600-401-hb9s

Der Kaninchen Polyklonal Anti-ERN1-Antikörper wurde für WB, ELISA, IHC, IF und FM validiert. Er ist geeignet, ERN1 in Proben von Human zu detektieren.

Kurzübersicht für ERN1 Antikörper (Internal Region) (ABIN7540596)

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Produktdetails anti-ERN1 Antikörper

Bindungsspezifität: Internal Region

Hersteller Produkt- Nr.: 600-401-hb9s

Hersteller: Rockland

Verwendungszweck: Ire1 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against mouse Ire1.

Aufreinigung: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen: Anti-Ire1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Ire1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000-1:50,000

Immunohistochemistry_Dilution: 1:100

IF_Microscopy_Dilution: 10 μg/mL

Western_Blot_Dilution: 1:1000

Kommentare:

Anti-Ire1 Antibody has been tested in ELISA, Western Blot, Immunofluorescence, and Immunohistochemistry. Expect a band in mouse for isoform 1 at ~110.2kDa and 2 at ~45.3kDa, and in human at ~109.7 kDa in western blot using appropriate lysates or tissues.  Positive control used: Mouse brain tissue in Western Blot,  SCLC-21H cells in IF, and Human stomach tissue in IHC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu ERN1

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Andere Bezeichnung: Ern1

Hintergrund: Rabbit Anti-Ern1 Antibody, Rabbit Anti-Endoplasmic Reticulum To Nucleus Signaling 1 Antibody, Rabbit Anti-IRE1 Antibody, Endoplasmic Reticulum To Nucleus Signaling 1, Serine/Threonine-Protein Kinase/Endoribonuclease IRE1, Inositol-Requiring Protein 1, Inositol-Requiring Enzyme 1, ER To Nucleus Signalling 1, Ire1-Alpha, HIRE1p, IRE1a, IRE1, Endoplasmic Reticulum-To-Nucleus Signaling 1, Protein Kinase/Endoribonuclease, Inositol-Requiring 1, IRE1P,Inositol-requiring enzyme 1 (IRE1) or ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1) is a Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain, and subsequent activation of the endoribonuclease activity. The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA. The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes. Diseases associated with ERN1 include Chromosome 6Pter-P24 Deletion Syndrome and Alzheimer Disease. Anti-IRE1 Antibody is useful for researchers interested in Neuroscience research.

Gen-ID: 78943

NCBI Accession: NP_076402

UniProt: Q9EQY0

Pathways: ER-Nucleus Signaling, Unfolded Protein Response