Rekombinanter CCR5 Antikörper

Produktnummer ABIN7487681

Dieses Maus Chimeric-Antikörper erkennt spezifisch CCR5 in ELISA, FACS, Dep und SPR. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter CCR5 Antikörper (ABIN7487681)

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Chimeric

Konjugat: Dieser CCR5 Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS), Depletion (Dep), Surface Plasmon Resonance (SPR)

Klon: ST6

Produktdetails anti-CCR5 Antikörper

Verwendungszweck: Anti-CCR5 [ST6], Mouse IgG1, kappa

Spezifität: This antibody is specific for the YTSE region of the N-terminal extracellular domain of human CCR5.It also binds to rhesus CCR5.

Kreuzreaktivität: Rhesusaffen

Produktmerkmale:

Original Species of Ab: Rabbit

Original Format of Ab: IgG

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was isolated from a phage displayed antibody fragment library. The library was generated from a New Zealand White rabbit immunised with purified CCR5-N-GST protein, and four rounds of panning were completed against immobilised CCR5-N-MBP antigen.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: When expressed intracellularly, this antibody is able to inhibit the surface expression of human CCR5, conferring T-lymphocytes with immunity to CCR5-dependent HIV-1 infection (Steinberger et al, 2000). Humanised ST6 (ST6/34) also has the same depleting effect (Steinberger et al, 2000). The high affinity of this antibody for CCR5-N-MBP has been confirmed in surface plasmon resonance analysis (Steinberg et al, 2000). This antibody has been used to stain CCR5-expressing cells in flow cytometry, with no staining of control cells observed (Steinberger et al, 2000).

Kommentare:

This chimeric mouse antibody was made using the variable domain sequences of the original Rabbit IgG format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Andere Bezeichnung: CCR5

Hintergrund: CD195, C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CHEMR13, HIV-1 fusion coreceptor, B8LFP0

UniProt: P51681

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size