COL3 Antikörper

Produktnummer ABIN7478854

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch COL3 in WB, ELISA, IF, IHC (p) und RIA. Es zeigt Reaktivität gegenüber Proben von Huhn.

Kurzübersicht für COL3 Antikörper (ABIN7478854)

Target: COL3 (Collagen, Type III (COL3))

Reaktivität: Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COL3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Produktdetails anti-COL3 Antikörper

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte

Kreuzreaktivität (Details): Chicken, cross-reaction with human, bovine, mouse and rat collagen type III <0,1 %

Produktmerkmale: Type III collagen is an alpha1(III)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Chicken collagen type III 100 % , chicken collagen type I <5 %.

Aufreinigung: Affinity purified antibody

Immunogen: Purified collagen type III from chicken skin

Anwendungsinformationen

Applikationshinweise: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Chicken skin or liver,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA 1:40, IHC(P) 1:500, ELISA > 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Lyophilized from phosphate buffered solution, no BSA and preservative added!

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Details zu COL3

Target: COL3 (Collagen, Type III (COL3))

Andere Bezeichnung: Collagen Type III