Rekombinanter Topoisomerase II alpha Antikörper (AA 1352-1493)

Produktnummer ABIN7470682

Dieser Anti-Topoisomerase II alpha Antikörper ist ein Kaninchen Monoklonal Antikörper zur Detektion von Topoisomerase II alpha in IHC. Geeignet für Human.

Kurzübersicht für Rekombinanter Topoisomerase II alpha Antikörper (AA 1352-1493) (ABIN7470682)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: 802R

Produktdetails anti-Topoisomerase II alpha Antikörper

Bindungsspezifität: AA 1352-1493

Verwendungszweck: Topoisomerase 2-alpha / TOPO2A antibody (802R)

Immunogen: Recombinant human Topoisomerase II alpha fragment (aa1352-1493) (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Colon: A fraction of cells, especially in the crypt basis should show a moderate to strong nuclear TOPO2A immunostaining.

Product Description: Topoisomerase 2-alpha / TOPO2A antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Colon: Epithelial cells must not show any TOPO2A staining in the cytoplasm and in most nuclei of the surface epithelium.

Protokoll: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.

Haltbarkeit: 24 months

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: Topoisomerase 2-alpha

Hintergrund: Gene Alias: ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase II 170kD, DNA topoisomerase II alpha, Topoisomerase DNA II alpha 170 kDa, TP2A

UniProt: P11388

Pathways: Zellzyklus, Mitotic G1-G1/S Phases