Rekombinanter Prostate Specific Antigen Antikörper (AA 150-250)
Kurzübersicht für Rekombinanter Prostate Specific Antigen Antikörper (AA 150-250) (ABIN7477569)
Target
Alle Prostate Specific Antigen (PSA) Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
-
-
Bindungsspezifität
- AA 150-250
-
Immunogen
- Recombinant fragment (around aa150-250) of human KLK3 protein (exact sequence is proprietary)
-
Isotyp
- IgG
-
-
-
-
Applikationshinweise
- IHC 1:100-1:200
-
Kommentare
-
Positive Control: Prostate: The epithelial cells of the prostate glands must show a strong cytoplasmic staining. Due to leakage of the antigen in the vicinity of the prostate glands, adjacent stroma cells may display a weak to moderate staining reaction.
Negative Control: Colon: PSA immunostaining should be absent in all cells.
-
Protokoll
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Format
- Liquid
-
Lagerung
- 4 °C,-20 °C,-80 °C
-
Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
-
- Prostate Specific Antigen (PSA)
-
Andere Bezeichnung
- PSA
-
Hintergrund
- Antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin,PSA antibody validated for immunohistochemistry on 76 different Normal Tissues
-
UniProt
- P07288
-
Pathways
- Komplementsystem
Target
-