PAPPA Antikörper (AA 351-523)
Kurzübersicht für PAPPA Antikörper (AA 351-523) (ABIN7477551)
Target
Alle PAPPA Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- AA 351-523
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Immunogen
- Recombinant fragment (within aa 351-523) of human PAPP-A protein (exact sequence is proprietary)
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Isotyp
- IgG
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Applikationshinweise
- IHC 1:75-1:150
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Kommentare
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Positive Control: Placenta: Trophoplast and chorion cells should show a strong cytoplasmic papp-A staining.
Negative Control: Colon: Papp-A immunostaining should be completely absent in both stromal end epithelial cells.
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Protokoll
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))
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Andere Bezeichnung
- PAPP-A
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Hintergrund
- ASBABP2, Aspecific BCL2 ARE binding protein 2, Differentially placenta 1 expressed protein, DIPLA1, IGF-dependent, IGFBP4ase, Insulin-like growth factor-dependent IGF-binding protein 4 protease (IGFBP-4 protease), PAPA, PAPP A, PAPPA1, Pappalysin-1, Pregnancy Associated Plasma Protein A (PAPP-A),PAPP-A antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- Q13219
Target
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