Rekombinanter MSH6 Antikörper (AA 1-100)

Produktnummer ABIN7477533

Dieses Kaninchen Monoklonal-Antikörper erkennt spezifisch MSH6 in IHC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter MSH6 Antikörper (AA 1-100) (ABIN7477533)

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser MSH6 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-906R

Produktdetails anti-MSH6 Antikörper

Bindungsspezifität: AA 1-100

Immunogen: Recombinant fragment (around aa 1-100) of human MSH6 protein (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:75-1:150

Kommentare:

Positive Control: Tonsil: Virtually all mantle zone B-cells must show an at least weak to moderate nuclear staining. A moderate to strong nuclear staining must be seen in the germinal centre B-cells.

Negative Control: Colon adenocarcinoma with previously documented loss of MSH6 expression: Nuclear staining should be absent in cancer cells, while a nuclear staining must be seen in stromal and inflammatory cells.

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu MSH6

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Andere Bezeichnung: MSH6

Hintergrund: DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, mutS (E. coli) homolog 6, MutS alpha 160 kDa subunit, MutS-alpha 160 kDa subunit, p160, Sperm associated protein,MSH6 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P52701

Pathways: DNA Reparatur, Chromatin Binding, Production of Molecular Mediator of Immune Response