Rekombinanter MSH2 Antikörper

Produktnummer ABIN7477531

Dieses Anti-MSH2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von MSH2 in IHC. Geeignet für Human.

Kurzübersicht für Rekombinanter MSH2 Antikörper (ABIN7477531)

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MSH2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-902M

Produktdetails anti-MSH2 Antikörper

Immunogen: Recombinant full-length human MSH2 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Tonsil: Virtually all mantle zone B-cells must show an at least weak to moderate nuclear staining. A moderate to strong nuclear staining must be seen in the germinal centre B-cells.

Negative Control: Colon adenocarcinoma with previously documented loss of MSH2 expression: no nuclear staining should be seen in cancer cells, while a nuclear staining must be seen in stromal and inflammatory cells.

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Andere Bezeichnung: MSH2

Hintergrund: BAT26, COCA1, DNA mismatch repair protein Msh2, FCC1, hMSH2, HNPCC1, LCFS2, MSH2, MutS homolog 2, MutS homolog 2 colon cancer nonpolyposis type 1, MutS protein homolog 2,MSH2 antibody validated for immunohistochemistry on 76 different Normal Tissues

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response