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GAD65 Antikörper (AA 6-99)

Name: GAD65 Antikörper (AA 6-99)
Brand: antibodies-online
SKU: ABIN7477477
Price: 1433.01 EUR
Availability: InStock

GAD2 Reaktivität: Human IHC Wirt: Maus Monoclonal MSVA-602M unconjugated

Strong GAD2 staining of nerve fibres in the grey matter of the cerebrum (GAD65 Antikörper (AA 6-99))

IHC

Pancreatic neuroendocrine tumor with strong GAD2 immunostaining of tumor cells (GAD65 Antikörper (AA 6-99))

IHC

Strong cytoplasmic GAD2 staining of pancreatic islet cells (GAD65 Antikörper (AA 6-99))

IHC

3 Abbildungen

Produktnummer ABIN7477477

Datenblatt (PDF)

Target Alle GAD65 (GAD2) Antikörper anzeigen

GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
Bindungsspezifität
16

10

8

7

7

6

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 6-99
Reaktivität
86

59

34

16

1

1

1

1

1

1
Human
Wirt
83

29

1
Maus
Passende Sekundärantikörper
Klonalität
86

27
Monoklonal
Konjugat
63

11

9

4

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Dieser GAD65 Antikörper ist unkonjugiert
Applikation
86

37

33

17

17

17

15

14

13

13

4

3

2

2

1

1

1

1
Immunohistochemistry (IHC)

Immunogen

Recombinant human GAD2 (GAD65) protein fragment (around aa 6-99) (exact sequence is proprietary)

Klon

MSVA-602M

Isotyp

IgG2b, kappa

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Applikationshinweise

IHC 1:100-1:200

Kommentare

Positive Control: Pancreas: A strong GAD2 staining should be seen in pancreatic islet cells.
Negative Control: Colon: GAD2 immunostaining should be absent in all cell types

Protokoll

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Lagerung

4 °C,-20 °C,-80 °C

Informationen zur Lagerung

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
Target

GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Andere Bezeichnung

GAD2 (GAD2 Produkte)

Hintergrund

GAD65,GAD2 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt

Q05329