Rekombinanter Cytokeratin 18 Antikörper
Kurzübersicht für Rekombinanter Cytokeratin 18 Antikörper (ABIN7477447)
Target
Alle Cytokeratin 18 (KRT18) Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Immunogen
- Recombinant human full-length KRT18 protein
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Isotyp
- IgG
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Applikationshinweise
- IHC 1:100-1:200
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Kommentare
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Positive Control: Liver: At least a weak to moderate, predominantly membranous staining should be seen in virtually all hepatocytes. Appendix: A strong staining should be seen in all epithelial cells.
Negative Control: Appendix: Staining should be absent in all non-epithelial cells of the appendix.
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Protokoll
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
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Andere Bezeichnung
- Cytokeratin 18
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Hintergrund
- Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18,Cytokeratin 18 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P05783
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Pathways
- Apoptose, Caspase Kaskade in der Apoptose
Target
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