Rekombinanter CTLA4 Antikörper
Kurzübersicht für Rekombinanter CTLA4 Antikörper (ABIN7477429)
Target
Alle CTLA4 Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Isotyp
- IgG
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Applikationshinweise
- IHC 1:50-1:100
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Kommentare
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Positive Control: Tonsil: A moderate strong membranous and cytoplasmic CTLA4 immunostaining should be seen in a subset of T-lymphocytes with strongest staining in follicular T-helper cells of germinal centres.
Negative Control: Tonsil: The majority of lymphocytes in germinal centres and all epithelial cells should stain negative.
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Protokoll
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
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Andere Bezeichnung
- CTLA-4
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Hintergrund
- ALPS5, CD152, Celiac disease 3 (CELIAC3), Cytotoxic T-lymphocyte-associated antigen 4 (CTLA4), GRD4, GSE, ICOS, Insulin-dependent Diabetes Mellitus 12 (IDDM12),CTLA-4 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P16410
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Pathways
- Cancer Immune Checkpoints
Target
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