Rekombinanter CD3 Antikörper (AA 20-40)
Kurzübersicht für Rekombinanter CD3 Antikörper (AA 20-40) (ABIN7477391)
Target
Alle CD3 Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- AA 20-40
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Immunogen
- A synthetic peptide from human CD3e protein (around aa 20-40) (exact sequence is proprietary)
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Isotyp
- IgG
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anti-CD3 (CD3) antibody
CD3 Reaktivität: Human, Maus, Ratte IHC, IF, WB Wirt: Kaninchen Polyclonal unconjugated
anti-CD3 (CD3) antibodyCD3 Reaktivität: Human, Non-Human Primate FACS, IP, IHC (fro), ICC, MS Wirt: Maus Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibodyCD3 Reaktivität: Human, Non-Human Primate FACS, IP, IHC (fro), Func, MS Wirt: Maus Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibodyCD3 Reaktivität: Human FACS, IP, Func, MS Wirt: Maus Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibodyCD3 Reaktivität: Human FACS, IP, MS Wirt: Maus Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibody (Biotin)CD3 Reaktivität: Human, Non-Human Primate FACS, IP, IHC (fro) Wirt: Maus Monoclonal UCHT1 Biotin
anti-CD3 (CD3) antibody (Biotin)CD3 Reaktivität: Human FACS, IP Wirt: Maus Monoclonal MEM-57 Biotin
anti-CD3 (CD3) antibody (PerCP)CD3 Reaktivität: Human, Non-Human Primate FACS Wirt: Maus Monoclonal UCHT1 PerCP
anti-CD3 (CD3) antibody (APC)CD3 Reaktivität: Human, Non-Human Primate FACS Wirt: Maus Monoclonal UCHT1 APC
anti-CD3 (CD3) antibody (PE)CD3 Reaktivität: Human, Non-Human Primate FACS Wirt: Maus Monoclonal UCHT1 PE
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Applikationshinweise
- IHC 1:100-1:200
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Kommentare
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Positive Control: Tonsil: A moderate to strong membranous staining should be seen in all T-cells in the interfollicular T-zones and in the germinal centres (few cells).
Negative Control: Tonsil: All epithelial structures and B-cells should not show any CD3 immunostaining.
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Protokoll
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- CD3
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Andere Bezeichnung
- CD3
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Hintergrund
- CD3 epsilon, CD3 TCR complex, T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex,CD3 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P07766
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Pathways
- T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway
Target
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